ABSTRACT
Introduction: Propofol a short acting intravenous anaestheticis widely used with various adjuncts to facilitate improvedinsertion conditions of Proseal Laryneal Mask Airway(PLMA). We compared the median effective dose (ED 50)propofol requirement and insertion conditions of PLMA withsaline - propofol, dexmedetomidine - propofol and fentanyl -propofol.Material and Methods: This was a prospective randomizeddouble blind study conducted in 100 adult patients of AmericanSociety of Anaesthesiology (ASA) class I/II scheduled toundergo elective surgical procedures. They were randomlyallocated into four groups of 25 each - Group N normal saline,Group F1 fentanyl 1 mcg/kg, Group F2 fentanyl 2mcg/kg andGroup D dexmedetomidine 1mcg/kg. The study drug wasdiluted in 50ml saline and infused over 10 minutes followedby a predetermined dose of propofol as per Dixon’s up anddown method. The ease of PLMA insertion was assessed asper Muzi mouth opening score. Hemodynamic parameterswere observed starting from baseline (T0), at 10 min post testdrug infusion (T1), post propofol injection (T2), 1min postLMA insertion (T3) and till 3 minutes post LMA insertion(T4).Results: The ED50 of propofol for the insertion of PLMAwith normal saline, fentanyl (1mcg/kg), fentanyl (2 mcg/kg)and dexmedetomidine (1mcg/kg) as adjuncts were found outto be 3.25mg/kg, 2mg/kg, and 1.67mg/kg and 1.92mg/kgrespectively. PLMA insertion conditions and hemodynamicparameters were comparable between the four groups. Leastincidence of apnoea was noted in group D with only 8 patientsrequiring assisted ventilation.Conclusion: Dexmedetomidine significantly reduces therequirement of induction dose propofol for PLMA insertionwhile providing stable hemodynamic and excellent insertionconditions
ABSTRACT
Objective: To assess the functional outcome (clinical andradiological) of Variable angle hip screw (VHS) in trochantericfractures of femur.Materials and Methods: The study comprised of 30 patients,with follow up at regular intervals. According to Jensen andMichaelsen’s modification of Evan’s classification, 16 patientswere of stable patterns and 14 were of unstable patterns.Parameters which were evaluated during folow-up includedtime to partial weight bearing, time to full weight bearing,radiological union, mobility of the patient at 3 months and at 6months postoperatively.Results: Duration of surgery was <50 min in 14(46.7%)patients, 51-60 min in 11 (36.7%) patients in and >60 min in 5(16.6%) patients. Mean duration of surgery was 55 min. Rangeof duration of surgery was 48-78 min. In both stable andunstable fractures, majority of patients achieved partial weightbearing at 3-4 weeks and full weight bearing at 14-16 weeksand the difference was not significant. For Unstable groupRadiological union was achieved after 14 weeks in 50% ofpatients whereas in Stable group all patients achievedradiological union before 14 weeks and the difference wasstatistically significant (X2=12.293, df=3, p=0.008). Nosignificant difference was found among both the groups inmobility at 3 months and at 6 months. According to ModifiedSchatzker-Lambert score done at 6 months after surgery;excellent result occurred in 23 cases (76.7%) , good resultoccurred in 5 cases (16.7%),fair result occurred in 2 cases(6.6%)Difference in Modified Schatzker- Lambert Score forboth groups was statistically significant (X2=7.028, df=2,p=0.03).Conclusion: Variable angle hip screw (VHS) can beconsidered an effective method of treating trochantericfractures in terms of functional outcome.
ABSTRACT
Lectins, algal in particular, have immense potential for biomedical applications such as anti-HIV, antitumoral, antimicrobial, anti-inflammatory and antinociceptive activities. In this context, there is a growing interest among researchers on agglutinins from green algae. Here, we have made an attempt to catalogue lectins from various unexplored green algae species. Chlorophyceae members (Chlorella vulgaris, Chlorococcum infusiformis, Desmodesmus dimorphus, D. subspicatus and Scenedesmus quadricauda) were screened for lectin activity using human, pig, sheep, goat and rabbit erythrocytes. All of them showed surface bound lectin activity with highest agglutination titre towards human blood type B erythrocytes and rabbit erythrocytes. Neuraminidase and protease treatment to human blood type B erythrocytes considerably enhanced the agglutination titre of lectins from S. quadricauda, C. vulgaris and D. subspicatus. However, protease treatment of erythrocytes showed no effect on C. infusiformis lectin activity, and decreased the lectin activity of D. dimorphus. Lectins of members of chlorophyceae have shown unique glycoprotein binding specificities as their lectin activity was specifically inhibited by glycoproteins exhibiting complex O-glycans, such as bovine submaxillary mucin, porcine stomach mucin and fetuin. All the algal cultures expressed maximum lectin activity during stationary phase of growth except S. quadricauda which expressed maximum lectin activity during mid-log to stationary phase of cultivation. Possibly, it is a new report on cell surface bound lectins from unexplored members of chlorophyceae for lectin activity.
ABSTRACT
Treatment options for large osteoperiosteal defects are limited and that which are available are not ideal. Osteoperiosteal defect were created in ulnae of both forelimbs of rabbits and tricalcium phosphate implant was used to bridge the gap. Amongst the 35 implanted ulnae, one implant got dislodged. Rest of the implants showed good adherence to host bone until the final follow up. Five control rabbit limbs (in which no implants were put) showed persistent bone gap. Histological and Electron microscopic examination revealed bone tissues covering the surface of the implant and bridging the gap. New bone was formed in the pores also. Tricalcium phosphate implants showed new bone formation due to osteoconductive properties. They are biodegradable. It is suggested that tricalcium phosphate implants are viable treatment alternatives in management of large osteoperiosteal defects with minimal to no adverse effects.